Generator

Part:BBa_K539652:Design

Designed by: Shu-Han Chang   Group: iGEM11_NCTU_Formosa   (2011-09-27)

promoter (lacI regulated) + a RBS (B0032)+Alss with a RBS (B0032)+ilvC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2562
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2148
    Illegal BsaI.rc site found at 535
    Illegal BsaI.rc site found at 1129
    Illegal BsaI.rc site found at 2967


Design Notes

Built in Plac, two weak expressing RBSs, Alss and ilvC (Part:BBa_K539631 with Part:BBa_K539622). Both of Alss and ilvC had been mutated to delet the function of RE site at the origin so that we can submit this part to the iGEM.

Source

The sequence of Alss comes from Bacillus subtilis. However ,the ilvC is from the Escherichia coli str. K-12 substr. MG1655

References